Rat 14-3-3 Protein Theta (YWHAQ) ELISA Kit | YWHAQ elisa kit

Rat 14-3-3 Protein Theta (YWHAQ) ELISA Kit

Cat. Num. AAA9711599

• Reactivity: Rat
• Synonyms: 14-3-3 Protein Theta (YWHAQ); Rat 14-3-3 Protein Theta (YWHAQ) ELISA Kit; YWHAQ; 14-3-3; 1C5; HS1; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; protein tau; tyrosine 3/tryptophan 5-monooxygenase activation protein; theta polypeptide; YWHAQ elisa kit

• Reactivity: Rat
• Specificity: Rat 14-3-3 protein theta (YWHAQ) ELISA Kit has high sensitivity and excellent specificity for detection of Rat YWHAQ. No significant cross-reactivity or interference between Rat YWHAQ and analogues was observed.
• Sequence Length: 245

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat 14-3-3 protein theta (YWHAQ) Standard Curve.)

Related Product Information for YWHAQ elisa kit

Description: This Rat 14-3-3 protein theta (YWHAQ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245-amino acid protein has a calculated molecular mass of 28 kD and shares 72% amino acid identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.

NCBI and Uniprot Product Information

• NCBI GI #: 126723376
• NCBI GeneID: 100008930
• NCBI Accession #: NP_001075637.1
• NCBI GenBank Nucleotide #: NP_001075637.1
• UniProt Accession #: Q6Q6X0
• NCBI Official Full Name: 14-3-3 protein theta
• NCBI Official Symbol: YWHAQ
• NCBI Protein Information: 14-3-3 protein theta
• UniProt Protein Name: 14-3-3 protein theta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAQ

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 ().