Rat Vascular Endothelial Growth Factor C (VEGFC) DIY ELISA Kit | VEGFC diy elisa kit

Rat Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit DIY Materials

Cat. Num. AAA2088441

• Gene Names: VEGFC; VRP; Flt4-L; LMPH1D
• Reactivity: Rat
• Synonyms: Vascular Endothelial Growth Factor C (VEGFC); Rat Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit DIY Materials; VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein; VEGFC diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor C (VEGFC). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor C (VEGFC) and analogues was observed.
• Sequence Length: 419

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 15.62-1000pg/mL
• Sensitivity: 6.2pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for VEGFC diy elisa kit

Cytokine; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.4
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L; LMPH1D
• NCBI Protein Information: vascular endothelial growth factor C
• UniProt Protein Name: Vascular endothelial growth factor C
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: extracellular region; extracellular space

Molecular Function: chemoattractant activity; growth factor activity; protein binding; vascular endothelial growth factor receptor 3 binding

Biological Process: angiogenesis; induction of positive chemotaxis; platelet degranulation; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; response to hypoxia; signal transduction; substrate-bound cell migration; vascular endothelial growth factor receptor signaling pathway

Disease: Lymphedema, Hereditary, Id