Rat Matrix Gla Protein (MGP) DIY ELISA Kit | MGP diy elisa kit

Rat Matrix Gla Protein (MGP) ELISA Kit DIY Materials

Cat. Num. AAA2089104

• Gene Names: MGP; NTI; GIG36; MGLAP
• Reactivity: Rat
• Synonyms: Matrix Gla Protein (MGP); Rat Matrix Gla Protein (MGP) ELISA Kit DIY Materials; MGLAP; GIG36; Cell growth-inhibiting gene 36 protein; MGP diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Gla Protein (MGP). No significant cross-reactivity or interference between Matrix Gla Protein (MGP) and analogues was observed.
• Sequence Length: 103

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.28ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for MGP diy elisa kit

Metabolic pathway; Hematology; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 187591
• NCBI GeneID: 4256
• Molecular Weight: 15,325 Da
• NCBI Official Full Name: matrix Gla protein
• NCBI Official Synonym Full Names: matrix Gla protein
• NCBI Official Symbol: MGP
• NCBI Official Synonym Symbols: NTI; GIG36; MGLAP
• NCBI Protein Information: matrix Gla protein
• UniProt Protein Name: Matrix Gla protein
• Protein Family: Matrix Gla protein
• UniProt Gene Name: MGP
• UniProt Synonym Gene Names: MGLAP; MGP

NCBI Description

This gene encodes a member of the osteocalcin/matrix Gla family of proteins. The encoded vitamin K-dependent protein is secreted by chondrocytes and vascular smooth muscle cells, and functions as a physiological inhibitor of ectopic tissue calcification. Carboxylation status of the encoded protein is associated with calcification of the vasculature in human patients with cardiovascular disease and calcification of the synovial membranes in osteoarthritis patients. Mutations in this gene cause Keutel syndrome in human patients, which is characterized by abnormal cartilage calcification, peripheral pulmonary stenosis and facial hypoplasia. [provided by RefSeq, Sep 2016]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; protein binding; structural constituent of bone

Biological Process: cartilage condensation

Disease: Keutel Syndrome