Rat Glucagon Like Peptide 1 (GLP1) DIY ELISA Kit | GLP1 diy elisa kit

Rat Glucagon Like Peptide 1 (GLP1) ELISA Kit DIY Materials

Cat. Num. AAA2088813

• Gene Names: GCG; GLP1; GLP2; GRPP; GLP-1
• Reactivity: Rat
• Synonyms: Glucagon Like Peptide 1 (GLP1); Rat Glucagon Like Peptide 1 (GLP1) ELISA Kit DIY Materials; GLP1 diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Glucagon Like Peptide 1 (GLP1). No significant cross-reactivity or interference between Glucagon Like Peptide 1 (GLP1) and analogues was observed.
• Sequence Length: 180

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 12.35-1000pg/mL
• Sensitivity: 4.67pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for GLP1 diy elisa kit

Endocrinology; Cardiovascular biology; Hepatology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4503945
• NCBI GeneID: 2641
• NCBI Accession #: NP_002045.1
• NCBI GenBank Nucleotide #: NM_002054.4
• UniProt Accession #: P01275; Q53TP6; A6NN65
• Molecular Weight: 20,909 Da
• NCBI Official Full Name: glucagon preproprotein
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: GCG
• NCBI Official Synonym Symbols: GLP1; GLP2; GRPP; GLP-1
• NCBI Protein Information: glucagon
• UniProt Protein Name: Glucagon
• Protein Family: Germin-like protein
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2

NCBI Description

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Uniprot Description

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; plasma membrane

Molecular Function: glucagon receptor binding; hormone activity; identical protein binding; protein binding; receptor binding

Biological Process: cell proliferation; feeding behavior; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; regulation of insulin secretion; response to starvation; signal transduction