Rat Gastrin Releasing Peptide (GRP) DIY ELISA Kit | GRP diy elisa kit

Rat Gastrin Releasing Peptide (GRP) ELISA Kit DIY Materials

Cat. Num. AAA2089289

• Gene Names: GRP; BN; GRP-10; proGRP; preproGRP
• Reactivity: Rat
• Synonyms: Gastrin Releasing Peptide (GRP); Rat Gastrin Releasing Peptide (GRP) ELISA Kit DIY Materials; BN; GRP10; Bombesin; Neuromedin C; GRP diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Gastrin Releasing Peptide (GRP). No significant cross-reactivity or interference between Gastrin Releasing Peptide (GRP) and analogues was observed.
• Sequence Length: 138

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 12.35-1000pg/mL
• Sensitivity: 4.69pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for GRP diy elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 60498999
• NCBI GeneID: 2922
• NCBI Accession #: NP_001012531.1
• NCBI GenBank Nucleotide #: NM_001012513.2
• UniProt Accession #: P07492; P07491; P81553; Q14454; Q53YA0; Q9BSY7
• Molecular Weight: 15,533 Da
• NCBI Official Full Name: gastrin-releasing peptide isoform 3 preproprotein
• NCBI Official Synonym Full Names: gastrin releasing peptide
• NCBI Official Symbol: GRP
• NCBI Official Synonym Symbols: BN; GRP-10; proGRP; preproGRP
• NCBI Protein Information: gastrin-releasing peptide
• UniProt Protein Name: Gastrin-releasing peptide
• Protein Family: Gastrin-releasing peptide
• UniProt Gene Name: GRP
• UniProt Synonym Gene Names: GRP

NCBI Description

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. The encoded preproprotein is proteolytically processed to generate two peptides, gastrin-releasing peptide and neuromedin-C. These peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: extracellular region; extracellular space

Molecular Function: neuropeptide hormone activity

Biological Process: neuropeptide signaling pathway