Rat Fibrinogen Alpha (FGa) DIY ELISA Kit | FGa diy elisa kit

Rat Fibrinogen Alpha (FGa) ELISA Kit DIY Materials

Cat. Num. AAA2089024

• Gene Names: FGA; Fib2
• Reactivity: Rat
• Synonyms: Fibrinogen Alpha (FGa); Rat Fibrinogen Alpha (FGa) ELISA Kit DIY Materials; Fib2; FG-A; Fibrinopeptide A; FGa diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Fibrinogen Alpha (FGa). No significant cross-reactivity or interference between Fibrinogen Alpha (FGa) and analogues was observed.
• Sequence Length: 644

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Plasma.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.57ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for FGa diy elisa kit

Metabolic pathway; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 11761629
• NCBI GeneID: 2243
• NCBI Accession #: NP_068657.1
• NCBI GenBank Nucleotide #: NM_021871.3
• UniProt Accession #: P02671; Q4QQH7; Q9BX62; Q9UCH2; A8K3E4; D3DP14; D3DP15
• Molecular Weight: 69,757 Da
• NCBI Official Full Name: fibrinogen alpha chain isoform alpha
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: FGA
• NCBI Official Synonym Symbols: Fib2
• NCBI Protein Information: fibrinogen alpha chain
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGA

NCBI Description

This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. [provided by RefSeq, Jan 2016]

Uniprot Description

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.