Rat Prothrombin Fragment 1+2 (F1+2) CLIA Kit | F2 clia kit

Rat Prothrombin Fragment 1+2 (F1+2) CLIA Kit

Cat. Num. AAA2708837

• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Prothrombin Fragment 1+2 (F1+2); Rat Prothrombin Fragment 1+2 (F1+2) CLIA Kit; Prothrombin Fragment 1+2; F2 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2).; No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.
• Sequence Length: 617

• Applicable Applications for F2 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.55-400ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.23ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: Average the duplicate readings for each standard, control, and samples and subtract the average zero standard relative light unit (RLU). Create a standard curve on log-log graph paper, with F1+2 concentration on the y-axis and the RLU value on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, such as curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 135809
• NCBI GeneID: 29251
• UniProt Accession #: P18292
• NCBI Official Full Name: Prothrombin
• NCBI Official Synonym Full Names: coagulation factor II
• NCBI Official Symbol: F2
• NCBI Protein Information: prothrombin
• UniProt Protein Name: Prothrombin
• Protein Family: Prothrombin
• UniProt Gene Name: F2
• UniProt Entry Name: THRB_RAT

NCBI Description

catalyzes the preferential cleavage of Arg-Gly; activates fibrinogen to fibrin and releases fibrinopeptides A and B; involved in blood coagulation and wound repair [RGD, Feb 2006]

Uniprot Description

prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.5; Protease; Apoptosis; Secreted

Cellular Component: extracellular matrix; extracellular space

Molecular Function: peptidase activity; serine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: negative regulation of proteolysis; response to inactivity; platelet activation; positive regulation of blood coagulation; cytosolic calcium ion homeostasis; wound healing; positive regulation of collagen biosynthetic process; proteolysis; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; fibrinolysis; cell surface receptor linked signal transduction; regulation of gene expression; positive regulation of cell proliferation; negative regulation of astrocyte differentiation; response to wounding; acute-phase response; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of protein amino acid phosphorylation; blood coagulation