Rat Pleiotrophin (PTN) CLIA Kit | PTN clia kit

Rat Pleiotrophin (PTN) CLIA Kit

Cat. Num. AAA2708918

• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Pleiotrophin (PTN); Rat Pleiotrophin (PTN) CLIA Kit; Pleiotrophin; HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule; PTN clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pleiotrophin (PTN).; No significant cross-reactivity or interference between Pleiotrophin (PTN) and analogues was observed.
• Sequence Length: 168

• Applicable Applications for PTN clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 2.74-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 1.31pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pleiotrophin (PTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pleiotrophin (PTN) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 8394103
• NCBI Accession #: NP_058762.1
• NCBI GenBank Nucleotide #: NP_058762.1
• UniProt Accession #: P63090
• NCBI Official Full Name: pleiotrophin
• UniProt Protein Name: Pleiotrophin
• Protein Family: Pleiotrophin
• UniProt Gene Name: Ptn
• UniProt Synonym Gene Names: PTN; HBBM; HBGF-8; HB-GAM; HBNF; OSF-1

Uniprot Description

Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. Binds to cell-surface target proteins via their chondroitin sulfate groups (). Down-regulates PTPRZ1 activity (PubMed:16814777).