Rat Nerve Growth Factor (NGF) CLIA Kit | NGF clia kit

Rat Nerve Growth Factor (NGF) CLIA Kit

Cat. Num. AAA2708671

• Gene Names: Ngf; Ngfb; beta-NGF
• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Nerve Growth Factor (NGF); Rat Nerve Growth Factor (NGF) CLIA Kit; Nerve Growth Factor; NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor; NGF clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF).; No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.
• Sequence Length: 241

• Applicable Applications for NGF clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 8.23-6,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 3.13pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor (NGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nerve Growth Factor (NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nerve Growth Factor (NGF) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 1352495
• NCBI GeneID: 310738
• UniProt Accession #: P25427
• NCBI Official Full Name: Beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb; beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF
• UniProt Entry Name: NGF_RAT

Uniprot Description

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.