Rat Growth Differentiation Factor 2 (GDF2) CLIA Kit | GDF2 clia kit

Rat Growth Differentiation Factor 2 (GDF2) CLIA Kit

Cat. Num. AAA2000807

• Gene Names: GDF2; BMP9; HHT5; BMP-9
• Reactivity: Rat
• Synonyms: Growth Differentiation Factor 2 (GDF2); Rat Growth Differentiation Factor 2 (GDF2) CLIA Kit; BMP9; Bone Morphogenetic Protein 9; GDF2 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 2 (GDF2).; No significant cross-reactivity or interference between Growth Differentiation Factor 2 (GDF2) and analogues was observed.
• Sequence Length: 429

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 10.5pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 2 (GDF2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 2 (GDF2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF2 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 2 (GDF2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 2 (GDF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 2 (GDF2) level in the sample or standard.;

Product Categories/Family for GDF2 clia kit

Cytokine; Metabolic pathway; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 119571045
• NCBI GeneID: 2658
• UniProt Accession #: Q9UK05; Q5VSQ9; Q9Y571
• Molecular Weight: 47,320 Da
• NCBI Official Full Name: growth differentiation factor 2
• NCBI Official Synonym Full Names: growth differentiation factor 2
• NCBI Official Symbol: GDF2
• NCBI Official Synonym Symbols: BMP9; HHT5; BMP-9
• NCBI Protein Information: growth/differentiation factor 2
• UniProt Protein Name: Growth/differentiation factor 2
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF2
• UniProt Synonym Gene Names: BMP9; GDF-2; BMP-9
• UniProt Entry Name: GDF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jan 2014]

Uniprot Description

GDF2: Could be involved in bone formation. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; regulation of apoptosis; BMP signaling pathway; positive regulation of interleukin-8 production; positive regulation of endothelial cell differentiation; angiogenesis; vasculogenesis; positive regulation of BMP signaling pathway; ossification; negative regulation of blood vessel endothelial cell migration; osteoblast differentiation; patterning of blood vessels; positive regulation of osteoblast differentiation; negative regulation of angiogenesis; positive regulation of angiogenesis; negative regulation of DNA replication; cartilage development; regulation of MAPKKK cascade; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell development; growth

Disease: Telangiectasia, Hereditary Hemorrhagic, Type 5