Rat Epiregulin (EREG) CLIA Kit | EREG clia kit

Rat Epiregulin (EREG) CLIA Kit

Cat. Num. AAA2709007

• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Epiregulin (EREG); Rat Epiregulin (EREG) CLIA Kit; Epiregulin; EPR; ER; Proepiregulin; EREG clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Epiregulin (EREG).; No significant cross-reactivity or interference between Epiregulin (EREG) and analogues was observed.
• Sequence Length: 162

• Applicable Applications for EREG clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.48pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epiregulin (EREG) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epiregulin (EREG) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Epiregulin (EREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epiregulin (EREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epiregulin (EREG) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 11078689
• NCBI GeneID: 59325
• NCBI Accession #: NP_067721.1
• NCBI GenBank Nucleotide #: NP_067721.1
• UniProt Accession #: Q9Z0L5
• NCBI Official Full Name: proepiregulin preproprotein
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: Ereg
• NCBI Protein Information: proepiregulin
• UniProt Protein Name: Proepiregulin
• Protein Family: Proepiregulin
• UniProt Gene Name: Ereg
• UniProt Synonym Gene Names: EPR

NCBI Description

may play a role in vascular smooth muscle cell proliferation and vascular remodeling stimulated by vasoactive agonists [RGD, Feb 2006]

Uniprot Description

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9990076). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357).