BrdU Cell Proliferation Assay Kit
BrdU Cell Proliferation
Reactivity
BrdU Incorporated Cells
Applications
ELISA
Synonyms
BrdU Cell Proliferation; BrdU Cell Proliferation assay kit
Reactivity
BrdU Incorporated Cells
Applicable Applications for BrdU Cell Proliferation assay kit
ELISA (EIA)
Preparation and Storage
Store kit at -20 degree C until first use. Store at 4-8 degree C thereafter.
Related Product Information for BrdU Cell Proliferation assay kit
Intended Uses: The BrdU Cell Proliferation Assay Kit is a non-isotopic immunoassay for the quantitation of bromodeoxyuridine incorporation into newly synthesized DNA of actively proliferating cells.
Principle of the Assay: BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture 1X BrdU Reagent is added to wells of the microtiter plate. BrdU Reagent will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixative/Denaturing Solution. Prediluted Anti-BrdU Detector Antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU Reagent. Unbound Prediluted Anti-BrdU Detector Antibody is washed away and 1x Peroxidase Goat anti-Mouse IgG is added, which binds to the Prediluted Anti-BrdU Detector Antibody. The 1x Peroxidase Goat anti-Mouse IgG catalyzes the conversion of the TMB Substrate (chromogenic substrate tetra-methylbenzidine) from a colorless solution to a blue solution (or yellow after the addition of ELISA Stop Solution), the intensity of which is proportional to the amount of incorporated BrdU Reagent in the cells. The colored reaction product is quantified using a spectrophotometer.
Background/Introduction: The BrdU Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
Principle of the Assay: BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture 1X BrdU Reagent is added to wells of the microtiter plate. BrdU Reagent will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixative/Denaturing Solution. Prediluted Anti-BrdU Detector Antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU Reagent. Unbound Prediluted Anti-BrdU Detector Antibody is washed away and 1x Peroxidase Goat anti-Mouse IgG is added, which binds to the Prediluted Anti-BrdU Detector Antibody. The 1x Peroxidase Goat anti-Mouse IgG catalyzes the conversion of the TMB Substrate (chromogenic substrate tetra-methylbenzidine) from a colorless solution to a blue solution (or yellow after the addition of ELISA Stop Solution), the intensity of which is proportional to the amount of incorporated BrdU Reagent in the cells. The colored reaction product is quantified using a spectrophotometer.
Background/Introduction: The BrdU Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
Product Categories/Family for BrdU Cell Proliferation assay kit
Similar Products
Product Notes
The BrdU Incorporated Cells BrdU Cell Proliferation (Catalog #AAA396129) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA396129 Assay Kit recognizes BrdU Incorporated Cells BrdU Cell Proliferation. AAA Biotech's BrdU Cell Proliferation can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the BrdU Cell Proliferation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU Cell Proliferation, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
