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RAP, Rat Recombinant Protein | Rapgef3 recombinant protein

RAP, Recombinant, Rat (Receptor Associated Protein)

Gene Names
Rapgef3; Epac
Applications
Western Blot
Purity
Purified
>95% pure (SDS-PAGE)
Synonyms
RAP; Rat; Recombinant; Rat (Receptor Associated Protein); Rapgef3 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Purified
>95% pure (SDS-PAGE)
Form/Format
Supplied as a lyophilized powder from TBS, pH 7.5, 0.1% BSA, 0.09% sodium azide. Reconstitute with 100ul ddH2O.
Concentration
~1mg/ml (after reconstitution) (varies by lot)
Applicable Applications for Rapgef3 recombinant protein
Western Blot (WB)
Application Notes
Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, immunoblotting and receptor-binding studies. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Rat his:RAP
c-myc fusion partner.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short term only. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -70 degree C. Reconstituted product is stable for 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Rapgef3 recombinant protein
RAP binds with high affinity to certain members of the low-density lipoprotein (LDL) receptor family such as LDL receptor-related protein (LRP), gp330/megalin, and the very low-density lipoprotein (VLDL) receptor and, once bound to these receptors, antagonizes their ligand binding ability. Despite the presence of a putative signal sequence on RAP, this molecule is not secreted but remains cell-associated. RAP is contained primarily within the endoplasmic reticulum and Golgi compartments, with only a trace found at the cell surface and within the endosomal compartments. Genetic deletion of RAP in mice has no effect on LRP mRNA levels but does lead to incomplete processing of LRP in the brain and liver resulting in decreased LRP antigen levels in these organs.
Product Categories/Family for Rapgef3 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40kD
NCBI Official Full Name
rap guanine nucleotide exchange factor 3
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 3
NCBI Official Symbol
Rapgef3
NCBI Official Synonym Symbols
Epac
NCBI Protein Information
rap guanine nucleotide exchange factor 3; epac 1; cAMP-GEFI; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI)
UniProt Protein Name
Rap guanine nucleotide exchange factor 3
UniProt Gene Name
Rapgef3
UniProt Synonym Gene Names
Epac; Epac1; EPAC 1; cAMP-GEFI
UniProt Entry Name
RPGF3_RAT

NCBI Description

binds cAMP and activates the Ras superfamily guanine nucleotide binding protein (Rap1A)in a PKA-independent manner [RGD, Feb 2006]

Uniprot Description

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Cellular Component: membrane; cytoplasm; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; positive regulation of protein export from nucleus; positive regulation of angiogenesis; positive regulation of stress fiber formation; angiogenesis; regulation of protein kinase activity; positive regulation of peptidyl-serine phosphorylation; Rap protein signal transduction; positive regulation of GTPase activity

Research Articles on Rapgef3

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Product Notes

The Rapgef3 rapgef3 (Catalog #AAA636670) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAP, Rat can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, immunoblotting and receptor-binding studies. Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Rapgef3 rapgef3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP, Rat, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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