Ral B recombinant protein

Ral B

Cat. Num. AAA396490

• Reactivity: Human
• Applications: Affinity Binding
• Synonyms: Ral B; RalB Recombinant G-Protein; G protein; Ral B recombinant protein

• Reactivity: Human
• Form/Format: Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol
• Sequence Length: 206

• Applicable Applications for Ral B recombinant protein: Affinity Binding
• Immunogen: Recombinant GST protein from E. Coli
• Preparation and Storage: Product should be stored at -70 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for Ral B recombinant protein

RalB is a member of the Ras-like GTP binding protein family. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene. Ras and Ras-like proteins function as switches, cycling between 2 distinct conformational states, active when GTP is bound and inactive when GDP is bound. Affinity precipitation assays can be used to determine the activation status of Ras and Ras-like family members. This assay is based on the ability of the Ras family effector proteins to interact specifically with the activated GTP-bound form of their target GTPases.

Product Categories/Family for Ral B recombinant protein

Recombinant Protein; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4506405
• NCBI GeneID: 5899
• NCBI Accession #: NP_002872.1
• NCBI GenBank Nucleotide #: NM_002881.2
• UniProt Accession #: P11234; Q53T32
• Molecular Weight: 23,409 Da
• NCBI Official Full Name: ras-related protein Ral-B
• NCBI Official Synonym Full Names: v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
• NCBI Official Symbol: RALB
• NCBI Protein Information: ras-related protein Ral-B; RAS-like protein B
• UniProt Protein Name: Ras-related protein Ral-B
• UniProt Gene Name: RALB
• UniProt Entry Name: RALB_HUMAN

NCBI Description

This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis. Ref.12

Enzyme regulation: Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Subunit structure: Interacts with EXOC2 and EXOC8. Interacts with RALBP1 via its effector domain. Ref.9 Ref.10 Ref.14

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Note: During late cytokinesis localizes at the midbody. Ref.11 Ref.12

Post-translational modification: Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).

Sequence similarities: Belongs to the small GTPase superfamily. Ras family.