DNA Repair Protein RAD50 Recombinant Protein | RAD50 recombinant protein

Recombinant DNA Repair Protein RAD50 (RAD50)

Cat. Num. AAA2031094

• Gene Names: RAD50; NBSLD; RAD502; hRad50
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: DNA Repair Protein RAD50; Recombinant DNA Repair Protein RAD50 (RAD50); RAD50 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MSRIEKMSIL GVRSFGIEDK DKQIISFFSP LTILVGPNGA GKTTIIECLKYICTGDFPPG TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GEMVAVHRSMLCSQKNKKTE FKTLEGVITR MKHGEKVSLS SKCAEIDREM ISCLGVSKSVLNNVIFCHQE DSNWPLSEGK ALKQKFDEIF SATRYIKALD TLRQVRQTQGQKVKECQTEL KYLKQNKEKA CEIRDQITSK EAQL
• Sequence Length: 1312

• Applicable Applications for RAD50 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.2kDa
• Accurate Molecular Mass (KD): 30kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Leu234
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 19924129
• NCBI GeneID: 10111
• NCBI Accession #: NP_005723.2
• NCBI GenBank Nucleotide #: NM_005732.3
• Molecular Weight: 138,432 Da
• NCBI Official Full Name: DNA repair protein RAD50
• NCBI Official Synonym Full Names: RAD50 homolog, double strand break repair protein
• NCBI Official Symbol: RAD50
• NCBI Official Synonym Symbols: NBSLD; RAD502; hRad50
• NCBI Protein Information: DNA repair protein RAD50
• UniProt Protein Name: DNA repair protein RAD50
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD50
• UniProt Synonym Gene Names: hRAD50
• UniProt Entry Name: RAD50_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Uniprot Description

RAD50: a component of the Mre11-Rad50-NBS1 (MRN) complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Three alternatively spliced isoforms have been described.

Protein type: Hydrolase; DNA repair, damage; EC 3.6.-.-; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Mre11 complex; membrane; nuclear chromosome, telomeric region; pronucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding, bridging; protein binding; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; metal ion binding; 3'-5' exonuclease activity; ATP binding

Biological Process: meiotic recombination; double-strand break repair; positive regulation of kinase activity; telomere maintenance via telomerase; DNA repair; regulation of mitotic recombination; positive regulation of protein amino acid autophosphorylation; response to DNA damage stimulus; telomere maintenance; DNA catabolic process, endonucleolytic; DNA duplex unwinding; double-strand break repair via homologous recombination; DNA recombination

Disease: Nijmegen Breakage Syndrome-like Disorder