RAD1 recombinant protein

RAD1, 1-282aa, Human, His tag, E Coli

Cat. Num. AAA203563

• Gene Names: RAD1; REC1; HRAD1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RAD1; 1-282aa; Human; His tag; E Coli; Cell cycle checkpoint protein RAD1; HRAD1; REC1; RAD1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES
• Sequence Length: 282

• Applicable Applications for RAD1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAD1 recombinant protein

RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RAD1 recombinant protein

Cell Cycle

References

Liu Y., et al. (2010) J Biol Chem. 285 (8):5974-82.; Yazinski SA., et al. (2009) Proc Natl Acad Sci u S A. 106 (50):21282-7.;

NCBI and Uniprot Product Information

• NCBI GI #: 4506385
• NCBI GeneID: 5810
• NCBI Accession #: NP_002844
• NCBI GenBank Nucleotide #: NM_002853.3
• UniProt Accession #: O60671; O75572; O95304; Q1W161; Q5KSM0; Q5KSM1; Q9UEP1
• Molecular Weight: 33.9 kDa (302aa) confirmed by MALDI-TOF
• NCBI Official Full Name: cell cycle checkpoint protein RAD1
• NCBI Official Synonym Full Names: RAD1 checkpoint DNA exonuclease
• NCBI Official Symbol: RAD1
• NCBI Official Synonym Symbols: REC1; HRAD1
• NCBI Protein Information: cell cycle checkpoint protein RAD1
• UniProt Protein Name: Cell cycle checkpoint protein RAD1
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD1
• UniProt Synonym Gene Names: REC1; hRAD1

NCBI Description

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (PubMed:9660799).