Rabbit anti Monkey IgG (heavy and light chains) Secondary Antibody
Rabbit anti Monkey IgG (heavy and light chains)
Applications
ELISA, Immunoblot, Western Blot
Synonyms
Rabbit anti Monkey IgG (heavy and light chains); Rabbit anti Monkey IgG (heavy and light chains) secondary antibody
Host
Rabbit
Specificity
Purified IgG fraction of polyclonal Rabbit antiSerum to Monkey IgG, heavy and light chains
Form/Format
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Applicable Applications for Rabbit anti Monkey IgG (heavy and light chains) secondary antibody
Indirect immunofluorescence, ELISA (EIA), Dot blot, Immunoblotting.
Product
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Source
Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Species Reactivity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with IgG in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The product may also react with IgG of other species as has been observed for Chimpanzee and man.
Preparation and Storage
The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Related Product Information for Rabbit anti Monkey IgG (heavy and light chains) secondary antibody
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Product Categories/Family for Rabbit anti Monkey IgG (heavy and light chains) secondary antibody
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Product Notes
The Rabbit anti Monkey IgG (heavy and light chains) (Catalog #AAA571140) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rabbit anti Monkey IgG (heavy and light chains) can be used in a range of immunoassay formats including, but not limited to, Indirect immunofluorescence, ELISA (EIA), Dot blot, Immunoblotting. Researchers should empirically determine the suitability of the Rabbit anti Monkey IgG (heavy and light chains) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabbit anti Monkey IgG (heavy and light chains), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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