Rabbit Nitric Oxide Synthase 2, Inducible (NOS2) RTU ELISA Kit | NOS2 rtu elisa kit

Rabbit Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit

Cat. Num. AAA4501409

• Gene Names: NOS2; NOS2A
• Reactivity: Rabbit
• Synonyms: Nitric Oxide Synthase 2; Inducible (NOS2); Rabbit Nitric Oxide Synthase 2; Inducible (NOS2) ELISA Kit; NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2 rtu elisa kit

• Reactivity: Rabbit
• Sequence Length: 496

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.54ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOS2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOS2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NOS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NOS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NOS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 8473491
• NCBI GeneID: 100008833
• UniProt Accession #: O19114
• Molecular Weight: 56,397 Da
• NCBI Official Full Name: Nitric oxide synthase, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS2A
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: Inducible NOS; iNOS

Uniprot Description

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.