Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-Pyk2-Y402 antibody at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human Pyk2-Y402 Antibody | anti-PTK2B antibody
Phospho-Pyk2-Y402
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-Pyk2-Y402 antibody at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-Pyk2-Y402 antibody at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.
Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Nuclear receptor co-regulator; TK group; Fak family
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: focal adhesion; postsynaptic density; dendrite; cell cortex; cytosol; lipid raft; N-methyl-D-aspartate selective glutamate receptor complex; nucleoplasm; extrinsic to internal side of plasma membrane; cytoskeleton; growth cone; cell soma; axon; perinuclear region of cytoplasm; lamellipodium; cytoplasm; nucleus
Molecular Function: protein binding; signal transducer activity; calmodulin-dependent protein kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; protein-tyrosine kinase activity; 3-phosphoinositide-dependent protein kinase binding; protein complex binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding
Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; regulation of cell shape; negative regulation of bone mineralization; regulation of inositol trisphosphate biosynthetic process; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; response to glucose stimulus; protein complex assembly; negative regulation of neuron apoptosis; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; response to osmotic stress; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; response to cAMP; peptidyl-tyrosine phosphorylation; blood vessel endothelial cell migration; positive regulation of translation; apoptosis; negative regulation of potassium ion transport; protein amino acid autophosphorylation; response to lithium ion; response to hormone stimulus; positive regulation of JNK cascade; regulation of calcium-mediated signaling; signal transduction; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; response to stress; angiogenesis; positive regulation of cell-matrix adhesion; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; stress fiber formation; response to hypoxia; innate immune response; cellular defense response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration
Research Articles on PTK2B
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Product Notes
The PTK2B ptk2b (Catalog #AAA9125156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Pyk2-Y402 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pyk2-Y402 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PTK2B ptk2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyk2-Y402, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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