Dot Blot
(Dot Blot of Rhodamine Conjugated Rabbit-anti-Mouse IgG1. Antigen: Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Rabbit-a-Mouse IgG1 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.)
Rabbit anti Mouse IgG1 (rhodamine) Secondary Antibody | IgG1 secondary antibody
Rabbit anti Mouse IgG1 (rhodamine)
Reactivity
Murine IgG1
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Rabbit anti Mouse IgG1 (Rhodamine) was purified by affinity chromatography.
Synonyms
Rabbit anti Mouse IgG1 (rhodamine); Rabbit anti Mouse Immunoglobulin G1 secondary (Rhodamine); IgG1 secondary antibody
Host
Rabbit
Reactivity
Murine IgG1
Specificity
Murine IgG1
Purity/Purification
Rabbit anti Mouse IgG1 (Rhodamine) was purified by affinity chromatography.
Form/Format
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Applicable Applications for IgG1 secondary antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
ELISA: 1:10,000-1:50,000
FC: 1:500-1:2,500
IF: 1:1,000-1:5,000
FC: 1:500-1:2,500
IF: 1:1,000-1:5,000
Biological Significance
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate
Rhodamine
Immunogen
Rabbit anti-mouse IgG1 (Rhodamine) was raised in rabbit using murine IgG1 heavy chain as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Dot Blot
(Dot Blot of Rhodamine Conjugated Rabbit-anti-Mouse IgG1. Antigen: Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Rabbit-a-Mouse IgG1 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.)
Dot Blot
(Dot Blot of Rhodamine Conjugated Rabbit-anti-Mouse IgG1. Antigen: Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Rabbit-a-Mouse IgG1 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.)
Related Product Information for IgG1 secondary antibody
Rabbit anti Mouse IgG1 secondary antibody (Rhodamine)
Product Categories/Family for IgG1 secondary antibody
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Product Notes
The IgG1 (Catalog #AAA538760) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Mouse IgG1 (rhodamine) reacts with Murine IgG1 and may cross-react with other species as described in the data sheet. AAA Biotech's Rabbit anti Mouse IgG1 (rhodamine) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF). ELISA: 1:10,000-1:50,000 FC: 1:500-1:2,500 IF: 1:1,000-1:5,000. Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabbit anti Mouse IgG1 (rhodamine), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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