Rabbit anti-Rat, Mouse Collagen Type III Antibody | anti-Col3a1 antibody
Collagen Type III, rat
Reactivity
Rat, Mouse
Applications
Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay
Purity
Affinity Purified
Synonyms
Collagen Type III; rat; anti-Col3a1 antibody
Host
Rabbit
Reactivity
Rat, Mouse
Purity/Purification
Affinity Purified
Form/Format
Lyophilized from phosphate buffered solution; no BSA and preservative added.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1463
Applicable Applications for anti-Col3a1 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA)
Application Notes
IFA: 1:80
IHC-P: 1:500
ELISA: 1:200
IHC-P: 1:500
ELISA: 1:200
Immunogen
Purified collagen type III from rat skin
Positive Control
Rat skin or liver
Incubation Time
IHC(P) 60 min at RT or 2-8 degree C over night
Pre-Treatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Preparation and Storage
Store at -20 degree C.
Related Product Information for anti-Col3a1 antibody
Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type III 100%, rat collagen I <0.1%; rat collagen type V <10%; rat elastin <0.1% (solid phase RIA at 1:50 dilution).
References
1. Bouvier M., Couble M.L., Hartmann D.J., Magloire H. (1991) Isolation and characterization of rat alveolar bone cells. Cell Mol. Biol. 37, 509-517. 2. Breen E., Falco V.M., Absher M., Cutroneo K.R. (1990) Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs. J. Biol. Chem. 265, 6286-6290. 3. Vialle-Presles M.J., Hartmann D.J., Franc S., Herbage D. (1989) Immunohistochemical study of the biological fate of a subcutaneous bovine collagen implant in rat. Histochemistry 91, 177-184. 4. Al Adnani M.S. (1989) Differential immunohistochemical localization of cytokeratins and collagen types I and III in experimentally-induced cirrhosis. J. Pathol. 159, 151-158.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
138,936 Da
NCBI Official Full Name
collagen alpha-1(III) chain
NCBI Official Synonym Full Names
collagen type III alpha 1 chain
NCBI Official Symbol
Col3a1
NCBI Protein Information
collagen alpha-1(III) chain
UniProt Protein Name
Collagen alpha-1(III) chain
Protein Family
UniProt Gene Name
Col3a1
NCBI Description
may be involved in craniofacial development [RGD, Feb 2006]
Uniprot Description
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 ().
Research Articles on Col3a1
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Product Notes
The Col3a1 col3a1 (Catalog #AAA397130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type III, rat reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type III can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA). IFA: 1:80 IHC-P: 1:500 ELISA: 1:200. Researchers should empirically determine the suitability of the Col3a1 col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type III, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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