Thrombomodulin, Rabbit Active Protein | THBD active protein
Thrombomodulin, Rabbit (CD141, TM, Fetomodulin
Purity
Purified95% by SDS-PAGE. Thrombomodulin is isolated from rabbit lung by a modification (3) of the procedure described by Galvin et al. (4). Purity is determined by SDS-PAGE analysis and activity is determined using a chromogenic assay for protein C ac
Synonyms
Thrombomodulin; Rabbit; Rabbit (CD141; TM; Fetomodulin; THBD active protein
Host
Rabbit lung
Purity/Purification
Purified
95% by SDS-PAGE. Thrombomodulin is isolated from rabbit lung by a modification (3) of the procedure described by Galvin et al. (4). Purity is determined by SDS-PAGE analysis and activity is determined using a chromogenic assay for protein C ac
95% by SDS-PAGE. Thrombomodulin is isolated from rabbit lung by a modification (3) of the procedure described by Galvin et al. (4). Purity is determined by SDS-PAGE analysis and activity is determined using a chromogenic assay for protein C ac
Form/Format
Supplied as a liquid in 0.02M Tris, 0.15M sodium chloride, pH 7.4, 0.05% polidocanol (PDOC).
Specific Activity
~500 U/mg
Extinction Coefficient
E1%1cm, 280nm=8.8 (5)
Isoelectric Point
2.5 (2)
Mode of Action
Cofactor; enhances the proteolytic activation of protein C by thrombin
Structure
Single chain, NH2-terminal hydrophobic domain, six EGF domains, one O-glycosylation rich domain, one transmembrane domain, COOH-terminal cytoplasmic domain
Post-translational Modifications
b-hydroxyasparagine (0.5 mol/mol of protein) (6)
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Related Product Information for THBD active protein
Thrombomodulin (TM) is an integral membrane glycoprotein expressed on the surface of endothelial cells. Its discovery by Esmon and Owen has focused attention on the importance of the protein C anticoagulant pathway (1,2). TM serves as a cofactor for protein C activation by forming a 1:1 stoichiometric complex with thrombin (Kd=10e-10M), which accelerates the rate of protein C activation by 1000-fold relative to the rate with thrombin alone. In addition to facilitating protein C activation, the binding of thrombin to TM drastically alters the procoagulant activity of thrombin. When bound to TM, thrombin no longer clots fibrinogen, activates factor V, inactivates protein S or triggers platelet aggregation.
TM is a single chain protein composed of 5 distinct domains. The domain structure of TM is similar to the low density lipoprotein (LDL) receptor. A short cytoplasmic domain containing a free cysteine is located at the COOH-terminal end and is joined by a membrane spanning region to an O-glycosylation rich domain. The latter is followed by an epidermal growth factor (EGF) homology region and the NH2-terminal hydrophobic domain. The EGF homology region contains 6 EGF-like domains and contains the binding sites for both thrombin and protein C.
TM is a single chain protein composed of 5 distinct domains. The domain structure of TM is similar to the low density lipoprotein (LDL) receptor. A short cytoplasmic domain containing a free cysteine is located at the COOH-terminal end and is joined by a membrane spanning region to an O-glycosylation rich domain. The latter is followed by an epidermal growth factor (EGF) homology region and the NH2-terminal hydrophobic domain. The EGF homology region contains 6 EGF-like domains and contains the binding sites for both thrombin and protein C.
Product Categories/Family for THBD active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74kD
NCBI Official Full Name
thrombomodulin
NCBI Official Symbol
THBD
NCBI Protein Information
thrombomodulin
Protein Family
Research Articles on THBD
Similar Products
Product Notes
The THBD (Catalog #AAA634741) is an Active Protein produced from Rabbit lung and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thrombomodulin, Rabbit, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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