Testing Data
Rabbit Tubulin gamma Antibody | anti-Tubulin gamma antibody
Tubulin Gamma Epitope Specific Rabbit antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The rabbit antibody is purified by affinity purification.
Synonyms
Tubulin gamma; Tubulin Gamma Epitope Specific Rabbit antibody; Rabbit anti-Tubulin gamma; Tubulin-gamma1; 2; GCP1; MGC131994; TUBG; TUBG1; Xgam; anti-Tubulin gamma antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human. The other species are not tested.
Purity/Purification
The rabbit antibody is purified by affinity purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-Tubulin gamma antibody
Western Blot, Immunohistochemistry
Application Notes
Western Blot: 1-2 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Antigen Preparation
A synthetic peptide corresponding to aa 432-451 of human tubulin.
Positive Control
Hela cells, Colon carcinoma
Cellular Location
Cytoplasm and nucleus
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-Tubulin gamma antibody
Tubulin gamma, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). It is an essential structural protein for cell growth organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma-depleted cells fail to form functional spindles and arrest during nuclear division. The phosphorylation of Tubulin gamma at Tyr445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings. Gamma tubulin 1 and Gamma tubulin 2 are different isoforms that are 97% homologous.
References
Zheng Y et al. Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex. Nature 378:578-83 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50 kDa
NCBI Official Full Name
tubulin gamma
NCBI Official Symbol
TUB4
NCBI Protein Information
tubulin gamma
Protein Family
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Product Notes
The Tubulin gamma (Catalog #AAA462217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tubulin Gamma Epitope Specific Rabbit antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunohistochemistry. Western Blot: 1-2 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100. Researchers should empirically determine the suitability of the Tubulin gamma for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin gamma, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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