Western Blot (WB)
(Western blot analysis of extracts from various samples, using S100 A10 Ab.Lane 1: Mouse lung lysates;Lane 2: Rat lung lysates;Lane 3: Rat lung lysates treated with blocking peptide;)
Rabbit S100A10 Antibody | anti-S100A10 antibody
S100A10 Antibody
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using S100 A10 Ab.Lane 1: Mouse lung lysates;Lane 2: Rat lung lysates;Lane 3: Rat lung lysates treated with blocking peptide;)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using S100 A10 Ab.Lane 1: Mouse lung lysates;Lane 2: Rat lung lysates;Lane 3: Rat lung lysates treated with blocking peptide;)
Immunohistochemistry (IHC)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Immunohistochemistry (IHC)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq, Jul 2008]
Uniprot Description
S100A10: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase. Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with SCN10A. Belongs to the S-100 family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: lipid raft
Molecular Function: lipid binding; protein binding; protein homodimerization activity
Biological Process: lipid raft formation; membrane budding; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of stress fiber formation; protein heterotetramerization
Research Articles on S100A10
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Product Notes
The S100A10 s100a10 (Catalog #AAA9387384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S100A10 Antibody reacts with Human,Mouse,Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100A10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the S100A10 s100a10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A10, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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