Rabbit RPA2 Antibody | anti-RPA2 antibody

RPA2 Antibody

Cat. Num. AAA8501250

• Gene Names: RPA2; REPA2; RPA32; RP-A p32; RP-A p34
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: RPA2; RPA2 Antibody; REPA2; RPA32; anti-RPA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 174

• Applicable Applications for anti-RPA2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human RPA2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of A549cell lines, using RPA2 antibody.)

Related Product Information for anti-RPA2 antibody

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol alpha, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10, 12).

Product Categories/Family for anti-RPA2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231. 2. Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838. 3. Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218. 4. Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527. 5. Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-439. 6. Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323. 7. Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85. 8. Guo, S. et al. (2006) J Biol Chem 281, 21607-16. 9. Wu, X. et al. (2005) Oncogene 24, 4728-4735. 10. Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024. 11. Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437. 12. Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.

NCBI and Uniprot Product Information

• NCBI GI #: 554595586
• NCBI GeneID: 6118
• NCBI Accession #: NP_001273005.1
• NCBI GenBank Nucleotide #: NM_001286076.1
• UniProt Accession #: P15927; Q52II0; Q5TEI9; Q5TEJ5
• Molecular Weight: 29kDa
• NCBI Official Full Name: replication protein A 32 kDa subunit isoform 2
• NCBI Official Synonym Full Names: replication protein A2
• NCBI Official Symbol: RPA2
• NCBI Official Synonym Symbols: REPA2; RPA32; RP-A p32; RP-A p34
• NCBI Protein Information: replication protein A 32 kDa subunit
• UniProt Protein Name: Replication protein A 32 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA2
• UniProt Synonym Gene Names: REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34

NCBI Description

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Uniprot Description

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.