Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using Phospho-PDPK1-S241 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit PDPK1-S241 Antibody | anti-PDPK1 antibody
Phospho-PDPK1-S241 Polyclonal Antibody
IHC: 1:50 - 1:100
IF: 1:100 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using Phospho-PDPK1-S241 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using Phospho-PDPK1-S241 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-PDPK1-S241 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-PDPK1-S241 antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-PDPK1-S241 antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-PDPK1-S241 antibody.)
NCBI and Uniprot Product Information
Uniprot Description
PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.
Protein type: Kinase, protein; Protein kinase, AGC; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; PKB family
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nucleoplasm; focal adhesion; cell projection; membrane; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; phospholipase binding; 3-phosphoinositide-dependent protein kinase activity; kinase activity; protein kinase binding; ATP binding; insulin receptor binding; phospholipase activator activity
Biological Process: focal adhesion formation; regulation of mast cell degranulation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; synaptic transmission; regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; activation of protein kinase B; peptidyl-threonine phosphorylation; calcium-mediated signaling; hyperosmotic response; cellular response to insulin stimulus; T cell costimulation; negative regulation of toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; negative regulation of protein kinase activity; positive regulation of release of sequestered calcium ion into cytosol; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on PDPK1
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Product Notes
The PDPK1 pdpk1 (Catalog #AAA128565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PDPK1-S241 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDPK1-S241 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:100 - 1:200. Researchers should empirically determine the suitability of the PDPK1 pdpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDPK1-S241, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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