Rabbit ITGB3 Antibody | anti-ITGB3 antibody

ITGB3 Antibody

Cat. Num. AAA8501837

• Gene Names: ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ITGB3; ITGB3 Antibody; CD61; GP3A; GPIIIa; anti-ITGB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 788

• Applicable Applications for anti-ITGB3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: A synthetic peptide of human ITGB3
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of Placentacell line, using ITGB3 antibody.)

Related Product Information for anti-ITGB3 antibody

Integrins are heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1, 2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlaping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1, 2). alphaIIbeta3 and alphaVbeta3 are the two beta3 containing integrins which are prominently expressed in hematopoietic cells and angiogenic endothelic cells and perform adhesive functions in hemostasis, wound healing and angiogenesis (1, 4). Tyr773 and Tyr785 (usually referred to as Tyr747 and Tyr759 based on the chicken sequence) are phosphorylated upon ligand binding (5). Phosphorylation of these tyrosine residues is required for certain ligand-induced signaling (6). Thr779 (corresponding to Thr753 of the chicken sequence) of integrin beta3 in the platelet specific alphaIIbeta3 is phosphorylated by PKD and/or Akt, which may modulate integrin association with other signaling molecules (7).

Product Categories/Family for anti-ITGB3 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571. 2. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100. 3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298. 4. Shattil, S.J. et al. (1995) Thromb. Haemost. 74, 149-155. 5. Blystone, S.D. (2002) J. Biol. Chem. 277, 46886-46890. 6. Butler, B. et al. (2003) J. Biol. Chem. 278, 5264-5270. 7. Kirk, R.I. et al. (2000) J. BIol. Chem. 275, 30901-30906.

NCBI and Uniprot Product Information

• NCBI GI #: 47078292
• NCBI GeneID: 3690
• NCBI Accession #: NP_000203.2
• NCBI GenBank Nucleotide #: NM_000212.2
• UniProt Accession #: P05106; O15495; Q12806; Q13413; Q14648; Q16499; A0PJW2; D3DXJ8
• Molecular Weight: 87kDa
• NCBI Official Full Name: integrin beta-3
• NCBI Official Synonym Full Names: integrin subunit beta 3
• NCBI Official Symbol: ITGB3
• NCBI Official Synonym Symbols: GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• NCBI Protein Information: integrin beta-3
• UniProt Protein Name: Integrin beta-3
• Protein Family: Integrin
• UniProt Gene Name: ITGB3
• UniProt Synonym Gene Names: GP3A; GPIIIa

NCBI Description

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887).