Rabbit HP1beta Antibody | anti-HP1beta antibody

HP1beta Antibody

Cat. Num. AAA8501611

• Gene Names: CBX1; CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: HP1beta; HP1beta Antibody; CBX; HP1-BETA; HP1Hs-beta; HP1Hsbeta; M31; MOD1; p25beta; anti-HP1beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 185

• Applicable Applications for anti-HP1beta antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Fusion protein of human CBX1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using CBX1antibody.)

Related Product Information for anti-HP1beta antibody

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (alpha, beta, and gamma) are primarily associated with centromeric heterochromatin; however, HP1beta and gamma also localize to euchromatic sites in the genome (2, 3). HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin (4, 5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10, 11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation (12-14).

Product Categories/Family for anti-HP1beta antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Maison, C. and Almouzni, G. (2004) Nat. Rev. Mol. Cell Biol. 5, 296-304. 2. Minc, E. et al. (2000) Cytogenet. Cell Genet. 90, 279-284. 3. Nielsen, A.L. et al. (2001) Mol. Cell 7, 729-739. 4. Lachner, M. et al. (2001) Nature 410, 116-120. 5. Bannister, A.J. et al. (2001) Nature 410, 120-124. 6. Muchardt, C. et al. (2002) EMBO Rep. 3, 975-981. 7. Yamamoto, K. and Sonoda, M. (2003) Biochem. Biophys. Res. Commun. 301, 287-292. 8. Fuks, F. et al. (2003) Nucleic Acids Res. 31, 2305-2312. 9. Murzina, N. et al. (1999) Mol. Cell 4, 529-540. 10. Nielsen, S.J. et al. (2001) Nature 412, 561-565. 11. Ogawa, H. et al. (2002) Science 296, 1132-1136. 12. Minc, E. et al. (1999) Chromosoma 108, 220-234. 13. Zhao, T. et al. (2001) J. Biol. Chem. 276, 9512-9518. 14. Lomberk, G. et al. (2006) Nat. Cell Biol. 8, 407-415.

NCBI and Uniprot Product Information

• NCBI GI #: 187960037
• NCBI GeneID: 10951
• NCBI Accession #: NP_001120700.1
• NCBI GenBank Nucleotide #: NM_001127228.1
• UniProt Accession #: P83916; P23197
• Molecular Weight: 27kDa
• NCBI Official Full Name: chromobox protein homolog 1
• NCBI Official Synonym Full Names: chromobox 1
• NCBI Official Symbol: CBX1
• NCBI Official Synonym Symbols: CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
• NCBI Protein Information: chromobox protein homolog 1
• UniProt Protein Name: Chromobox protein homolog 1
• UniProt Gene Name: CBX1
• UniProt Synonym Gene Names: CBX; HP1 beta

NCBI Description

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.