Western Blot (WB)
(Western blot analysis of extracts of 293 cells, using Phospho-GSK3B-Y216 antibody. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit GSK3B-Y216 Antibody | anti-GSK3B antibody
Phospho-GSK3B-Y216 Polyclonal Antibody
IHC: 1:50 - 1:100
IF: 1:100 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of 293 cells, using Phospho-GSK3B-Y216 antibody. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts of 293 cells, using Phospho-GSK3B-Y216 antibody. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-GSK3B-Y216 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-GSK3B-Y216 antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-GSK3B-Y216 antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-GSK3B-Y216 antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Uniprot Description
GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.26; EC 2.7.11.1; Kinase, protein; CMGC group; GSK family; GSK subfamily
Chromosomal Location of Human Ortholog: 3q13.3
Cellular Component: centrosome; dendritic spine; cytosol; beta-catenin destruction complex; ribonucleoprotein complex; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus
Molecular Function: p53 binding; beta-catenin binding; protein kinase binding; integrin binding; NF-kappaB binding; protein serine/threonine kinase activity; ionotropic glutamate receptor binding; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding
Biological Process: fat cell differentiation; circadian rhythm; glycogen metabolic process; axon guidance; negative regulation of MAP kinase activity; re-entry into mitotic cell cycle; genetic imprinting; nerve growth factor receptor signaling pathway; positive regulation of protein binding; positive regulation of axon extension; protein amino acid autophosphorylation; response to lithium ion; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; myoblast fusion; ER overload response; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; negative regulation of neuron maturation; peptidyl-serine phosphorylation; organ morphogenesis; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity
Research Articles on GSK3B
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Product Notes
The GSK3B gsk3b (Catalog #AAA128531) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-GSK3B-Y216 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3B-Y216 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:100 - 1:200. Researchers should empirically determine the suitability of the GSK3B gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3B-Y216, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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