Rabbit ERK3 (Phospho-Ser189) Antibody | anti-ERK3 antibody

Rabbit ERK3 (Phospho-Ser189) Antibody

Cat. Num. AAA4547

• Gene Names: MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
• Reactivity: Human, mouse, rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Synonyms: ERK3 (Phospho-Ser189); Rabbit ERK3 (Phospho-Ser189) Antibody; EC 2.7.11.24; ERK-3; ERK3; Extracellular signal-regulated kinase 3; MAP kinase isoform p97; MAPK6; MK06; Mitogen-activated protein kinase 6; PRKM4; PRKM6; kinase ERK3; p55-MAPK; p97-MAPK; anti-ERK3 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Specificity: ERK3 (Phospho-Ser189) antibody detects endogenous levels of ERK3 only when phosphorylated at serine 189.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 721

• Applicable Applications for anti-ERK3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ERK3 around the phosphorylation site of serine 189 (H-L-Sp-E-G).
• All Sites: Human: Ser186; Mouse: Ser189; Rat: Ser189

Immunohistochemistry

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ERK3 (Phospho-Ser189) antibody.)

Western Blot

(Western blot analysis of extracts from mouse brain, using ERK3 (Phospho-Ser189) antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4506091
• NCBI GeneID: 5597
• NCBI Accession #: NP_002739.1
• NCBI GenBank Nucleotide #: NM_002748.3
• UniProt Accession #: Q16659; Q68DH4; Q8IYN8
• Molecular Weight: 82,681 Da
• NCBI Official Full Name: mitogen-activated protein kinase 6
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 6
• NCBI Official Symbol: MAPK6
• NCBI Official Synonym Symbols: ERK3; PRKM6; p97MAPK; HsT17250
• NCBI Protein Information: mitogen-activated protein kinase 6; ERK-3; MAPK 6; p97-MAPK; MAP kinase 6; MAP kinase isoform p97; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97
• UniProt Protein Name: Mitogen-activated protein kinase 6
• UniProt Gene Name: MAPK6
• UniProt Synonym Gene Names: ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
• UniProt Entry Name: MK06_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; ATP binding

Biological Process: MAPKKK cascade; signal transduction; cell cycle; protein amino acid phosphorylation