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Western Blot (WB) (Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CFL1-S3 antibody at 1:1000 dilution. NIH/3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.)

Rabbit CFL1-S3 Antibody | anti-CFL1 antibody

Phospho-CFL1-S3 Polyclonal Antibody

Gene Names
CFL1; CFL; HEL-S-15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
CFL1-S3; Phospho-CFL1-S3 Polyclonal Antibody; Phospho-CFL1-S3: CFL; anti-CFL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
166
Applicable Applications for anti-CFL1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:100 - 1:200
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S3 of human CFL1
Calculated Molecular Weight
19kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CFL1-S3 antibody at 1:1000 dilution. NIH/3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.)

Western Blot (WB) (Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CFL1-S3 antibody at 1:1000 dilution. NIH/3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-CFL1-S3 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-CFL1-S3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-CFL1-S3 antibody.)

Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-CFL1-S3 antibody.)
Related Product Information for anti-CFL1 antibody
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Product Categories/Family for anti-CFL1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
166
NCBI Official Full Name
Cofilin-1
NCBI Official Synonym Full Names
cofilin 1 (non-muscle)
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; HEL-S-15
NCBI Protein Information
cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15
UniProt Protein Name
Cofilin-1
Protein Family
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: axon guidance; platelet activation; actin filament depolymerization; positive regulation of actin filament depolymerization; response to virus; cytoskeleton organization and biogenesis; negative regulation of cell size; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; protein import into nucleus; establishment of cell polarity; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis

Research Articles on CFL1

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Product Notes

The CFL1 cfl1 (Catalog #AAA128535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CFL1-S3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CFL1-S3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:100 - 1:200. Researchers should empirically determine the suitability of the CFL1 cfl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFL1-S3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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