Rabbit CDKN1C Antibody | anti-CDKN1C antibody

CDKN1C Antibody

Cat. Num. AAA8502895

• Gene Names: CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: CDKN1C; CDKN1C Antibody; BWCR; BWS; KIP2; WBS; p57; anti-CDKN1C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 316

• Applicable Applications for anti-CDKN1C antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide of human CDKN1C
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of HeLacell lines, using CDKN1C antibody.)

Related Product Information for anti-CDKN1C antibody

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).p57 Kip2 (Cyclin-dependent kinase inhibitor 1C) functions as a tumor suppressor. Mutations of p57 Kip2 have been associated with numerous human malignancies as well as Beckwith-Wiedemann syndrome (BWS), characterized by an increased risk of childhood cancer. The amino-terminal CDK inhibitory domain, common to the family, binds to and inhibits CDK/cyclin complexes and restricts cell cycle progression (5). The unique central region of p57 Kip2 interactes with LIMK-1, a downstream effector of the Rho family of GTPases. By sequestering LIMK-1 in the nucleus, p57 Kip2 disrupts actin dynamics within cells and may be linked to its essential role in embryonic development (6). In addition, the carboxyl-terminal QT domain of p57KIP2 binds to and inhibits JNK/SAPK activity regulating cellular apoptosis and differentiation (7). Expression levels of human p57 Kip2 are more restricted then other CDK inhibitors (8) and are controlled by ubiquitination/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2. This effect is dependent on Thr310 (9). A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability (10).

Product Categories/Family for anti-CDKN1C antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Lloyd, R.V. et al. (1999) Am. J. Pathol. 154, 313-323. 2. Polyak, K. et al. (1994) Genes Dev. 8, 9-22. 3. Kato, J.Y. et al. (1994) Cell 79, 487-496. 4. Vlach, J. et al. (1997) EMBO J. 16, 5334-5344. 5. Pateras, I.S. et al. (2009) Mol Cancer Res 7, 1902-19. 6. Yokoo, T. et al. (2003) J Biol Chem 278, 52919-23. 7. Chang, T.S. et al. (2003) J Biol Chem 278, 48092-8. 8. Lee, M.H. et al. (1995) Genes Dev 9, 639-49. 9. Kamura, T. et al. (2003) Proc Natl Acad Sci U S A 100, 10231-6. 10. Ishida, N. et al. (2000) J Biol Chem 275, 25146-54.

NCBI and Uniprot Product Information

• NCBI GI #: 4557441
• NCBI GeneID: 1028
• NCBI Accession #: NP_000067.1
• NCBI GenBank Nucleotide #: NM_000076.2
• UniProt Accession #: P49918
• Molecular Weight: 32kDa
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1C isoform a
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1C
• NCBI Official Symbol: CDKN1C
• NCBI Official Synonym Symbols: BWS; WBS; p57; BWCR; KIP2; p57Kip2
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1C
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1C
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: CDKN1C
• UniProt Synonym Gene Names: KIP2

NCBI Description

This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.MiscellaneousPaternally imprinted, therefore most expression comes from the maternal allele.