Rabbit Cdc6 Antibody | anti-Cdc6 antibody

Cdc6 Antibody

Cat. Num. AAA8501166

• Gene Names: CDC6; CDC18L; HsCDC6; MGORS5; HsCDC18
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: Cdc6; Cdc6 Antibody; CDC6; CDC18L; HsCDC18; HsCDC6; anti-Cdc6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 560

• Applicable Applications for anti-Cdc6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:100; IF: 1:20-1:50
• Immunogen: Fusion protein of human Cdc6
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of HeLa andJurkat cells using CDC6 antibody.)

Related Product Information for anti-Cdc6 antibody

The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome, and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1, 2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase, and formation of the pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases cdk2 and cdc7. Cdc7, which is active only in complex with its regulatory subunit dbf4, phosphorylates MCM proteins bound to chromatin and allows binding of the replication factor cdc45 and DNA polymerase (3, 4).Replication licensing is controlled in part by the degradation of cdc6 in quiescent cells. Phosphorylation of cdc6 by cdk2 prevents its degradation, allowing pre-replication complexes to form (5). Cdc6 has recently been shown to play an important role in the intra-S-phase p21 Waf1/Cip1-dependent DNA damage response (6, 7). Both cdc6 and CDT1 are degraded by the ubiquitin proteasome pathway in response to DNA damage associated with re-replication (8).

Product Categories/Family for anti-Cdc6 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

7. Okuno, Y. et al. (2001) EMBO J 20, 4263-77. 8. McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56. 9. Bell, S.P. and Dutta, A. (2002) Annu Rev Biochem 71, 333-74. 10. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72. 11. Mailand, N. and Diffley, J.F. (2005) Cell 122, 915-26. 12. Kan, Q. et al. (2008) J Biol Chem 283, 17864-72. 13. Kan, Q. et al. (2008) Proc Natl Acad Sci USA 105, 4757-62. 14. Hall, J.R. et al. (2008) J Biol Chem 283, 25356-63.

NCBI and Uniprot Product Information

• NCBI GI #: 4502703
• NCBI GeneID: 990
• NCBI Accession #: NP_001245.1
• NCBI GenBank Nucleotide #: NM_001254.3
• UniProt Accession #: Q99741; Q8TB30
• Molecular Weight: 63kDa
• NCBI Official Full Name: cell division control protein 6 homolog
• NCBI Official Synonym Full Names: cell division cycle 6
• NCBI Official Symbol: CDC6
• NCBI Official Synonym Symbols: CDC18L; HsCDC6; MGORS5; HsCDC18
• NCBI Protein Information: cell division control protein 6 homolog
• UniProt Protein Name: Cell division control protein 6 homolog
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC6
• UniProt Synonym Gene Names: CDC18L; HsCdc18; HsCDC6

NCBI Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.