Immunofluorescence/Immunocytochemistry (IF/ICC)
Rabbit APPL1 Antibody | anti-APPL1 antibody
Rabbit Anti-APPL1
Reactivity
Mouse, Rat, Human
Applications
Immunoprecipitation, Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Unpurified
Synonyms
APPL1; Rabbit Anti-APPL1; Adaptor Protein Containing pH Domain antibody; Adaptor protein containing pH domain PTB domain and leucinezipper motif 1 antibody; Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody; AKT2INTERACTOR antibody; APPL 1 antibody; APPL1 antibody; DCC interacting protein 13 alpha antibody; DIP 13 alphaantibody; DIP13 alpha antibody; DIP13alpha antibody; KIAA1428 antibody; PTB domain and leucine zipper motif 1antibody; PTB Domain and Leucine Zipper Motif antibody; Signaling adaptor protein DIP13alphaantibody; anti-APPL1 antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human
Specificity
APPL1
Purity/Purification
Unpurified
Form/Format
Unpurified antiserum containing 0.1% sodium azide;
Applicable Applications for anti-APPL1 antibody
Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; WB: 1/500. Predicted molecular weight: 80 kDa. ICC/IF: 1/500.
Target Antigen
APPL1
Immunogen
Purified APPL fusion
Research Areas
AKT2 interaction; protein binding
Immunofluorescence/Immunocytochemistry (IF/ICC)
Immunofluorescence/Immunocytochemistry (IF/ICC)
References
Identification of a chromosome 3p14.321.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoproteinserine/threonine kinase AKT2 Yasuhiro Mitsuuchi et al (Oncogene,1999) PMID: 10490823 Mackrell JG & Cartee GD A novel method to measure glucose uptake and myosin heavy chain isoform expression of single fibers from rat skeletal muscle. Diabetes 61:995-1003 (2012). WB ; Rat PMID: 22396201 Mackrell JG et al. Fiber type-specific differences in glucose uptake by single fibers from skeletal muscles of 9- and 25-month-old rats. J Gerontol A Biol Sci Med Sci 67:1286-94 (2012) PMID: 23042591 Sharma N et al. Mechanisms for increased insulin-stimulated Akt phosphorylation and glucose uptake in fast- and slow-twitch skeletal muscles of calorie-restricted rats. Am J Physiol Endocrinol Metab 300:E966-78 (2011) PMID: 21386065 Sharma N et al. Insulin resistance for glucose uptake and Akt2 phosphorylation in the soleus, but not epitrochlearis, muscles of old vs. adult rats. J Appl Physiol 108:1631-40 (2010) PMID: 20339009
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Product Notes
The APPL1 (Catalog #AAA375210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-APPL1 reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's APPL1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. Optimal antibody dilution should be determined by titration, however as a guideline try; WB: 1/500. Predicted molecular weight: 80 kDa. ICC/IF: 1/500. Researchers should empirically determine the suitability of the APPL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APPL1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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