Western blot
(Western blot analysis EDEM3 using HeLa whole cell lysates.)
Rabbit anti-Human, Mouse EDEM3 Antibody | anti-EDEM3 antibody
EDEM3 Antibody
Gene Names
EDEM3; C1orf22
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
EDEM3; EDEM3 Antibody; alpha-1; 2-mannosidase EDEM3; C1orf22; EDEM 3; edem3; EDEM3_HUMAN; ER degradation enhancer; mannosidase alpha like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation enhancing alpha mannosidase like protein 3; ER degradation-enhancing alpha-mannosidase-like 3;; anti-EDEM3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Isotype
IgG
Specificity
EDEM3 Antibody detects endogenous levels of total EDEM3
Purity/Purification
Immunogen affinity purified
Concentration
1.0mg/ml (varies by lot)
Sequence
MSEAGGRGCGSPVPQRARWRLVAATAAFCLVSATSVWTAGAEPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPSNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYEEVEVLLSDKAKDRDPEMENEEQPSSENDSQNQSGEQISSSSQEVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQTSNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL
Sequence Length
948
Applicable Applications for anti-EDEM3 antibody
Western Blot (WB)
Application Notes
Optimal dilutions/concentrations should be determined by the end user.
Immunogen
A synthesized peptide derived from human EDEM3
Subcellular Location
Endoplasmic reticulum lumen
Similarity
Belongs to the glycosyl hydrolase 47 family.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western blot
(Western blot analysis EDEM3 using HeLa whole cell lysates.)
Western blot
(Western blot analysis EDEM3 using HeLa whole cell lysates.)
Related Product Information for anti-EDEM3 antibody
Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes, by catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2 in the N-glycans. Seems to have alpha 1,2-mannosidase activity (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105kDa
NCBI Official Full Name
ER degradation-enhancing alpha-mannosidase-like protein 3 isoform 1
NCBI Official Synonym Full Names
ER degradation enhancing alpha-mannosidase like protein 3
NCBI Official Symbol
EDEM3
NCBI Official Synonym Symbols
C1orf22
NCBI Protein Information
ER degradation-enhancing alpha-mannosidase-like protein 3
UniProt Protein Name
ER degradation-enhancing alpha-mannosidase-like protein 3
Protein Family
UniProt Gene Name
EDEM3
UniProt Synonym Gene Names
C1orf22
NCBI Description
Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008]
Uniprot Description
EDEM3: Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes. This process depends on mannose-trimming from the N-glycans. Seems to have alpha 1,2-mannosidase activity. Belongs to the glycosyl hydrolase 47 family.
Protein type: EC 3.2.1.113; Hydrolase
Chromosomal Location of Human Ortholog: 1q25.3
Cellular Component: endoplasmic reticulum
Molecular Function: alpha-mannosidase activity; mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
Biological Process: ER-associated protein catabolic process; N-glycan processing; unfolded protein response
Similar Products
Product Notes
The EDEM3 edem3 (Catalog #AAA9384638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EDEM3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EDEM3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the EDEM3 edem3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSEAGGRGCG SPVPQRARWR LVAATAAFCL VSATSVWTAG AEPMSREEKQ KLGNQVLEMF DHAYGNYMEH AYPADELMPL TCRGRVRGQE PSRGDVDDAL GKFSLTLIDS LDTLVVLNKT KEFEDAVRKV LRDVNLDNDV VVSVFETNIR VLGGLLGGHS LAIMLKEKGE YMQWYNDELL QMAKQLGYKL LPAFNTTSGL PYPRINLKFG IRKPEARTGT ETDTCTACAG TLILEFAALS RFTGATIFEE YARKALDFLW EKRQRSSNLV GVTINIHTGD WVRKDSGVGA GIDSYYEYLL KAYVLLGDDS FLERFNTHYD AIMRYISQPP LLLDVHIHKP MLNARTWMDA LLAFFPGLQV LKGDIRPAIE THEMLYQVIK KHNFLPEAFT TDFRVHWAQH PLRPEFAEST YFLYKATGDP YYLEVGKTLI ENLNKYARVP CGFAAMKDVR TGSHEDRMDS FFLAEMFKYL YLLFADKEDI IFDIEDYIFT TEAHLLPLWL STTNQSISKK NTTSEYTELD DSNFDWTCPN TQILFPNDPL YAQSIREPLK NVVDKSCPRG IIRVEESFRS GAKPPLRARD FMATNPEHLE ILKKMGVSLI HLKDGRVQLV QHAIQAASSI DAEDGLRFMQ EMIELSSQQQ KEQQLPPRAV QIVSHPFFGR VVLTAGPAQF GLDLSKHKET RGFVASSKPS NGCSELTNPE AVMGKIALIQ RGQCMFAEKA RNIQNAGAIG GIVIDDNEGS SSDTAPLFQM AGDGKDTDDI KIPMLFLFSK EGSIILDAIR EYEEVEVLLS DKAKDRDPEM ENEEQPSSEN DSQNQSGEQI SSSSQEVDLV DQESSEENSL NSHPESLSLA DMDNAASISP SEQTSNPTEN HETTNLNGEC TDLDNQLQEQ SETEEDSNPN VSWGKKVQPI DSILADWNED IEAFEMMEKD EL. It is sometimes possible for the material contained within the vial of "EDEM3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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