Western Blot (WB)
(Western blot analysis of extracts of HeLa and 293 cells, using Phospho-SMC1A-S957 antibody at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. 293 cells were treated by serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit anti-Human SMC1A-S957 Antibody | anti-SMC1A antibody
Phospho-SMC1A-S957 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of HeLa and 293 cells, using Phospho-SMC1A-S957 antibody at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. 293 cells were treated by serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa and 293 cells, using Phospho-SMC1A-S957 antibody at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. 293 cells were treated by serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using Phospho-SMC1A-S957 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using Phospho-SMC1A-S957 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-SMC1A-S957 antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-SMC1A-S957 antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by UV using 2.5ug Phospho-SMC1A-S957 antibody. Western blot was performed from the immunoprecipitate using Phospho-SMC1A-S957 antibody at a dilition of 1:1000.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by UV using 2.5ug Phospho-SMC1A-S957 antibody. Western blot was performed from the immunoprecipitate using Phospho-SMC1A-S957 antibody at a dilition of 1:1000.)
NCBI and Uniprot Product Information
NCBI Description
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.
Protein type: DNA replication; RNA splicing
Chromosomal Location of Human Ortholog: Xp11.22-p11.21
Cellular Component: kinetochore; nucleoplasm; condensed nuclear chromosome; cohesin core heterodimer; cytoplasm; nucleolus; chromosome; cytosol; nucleus; chromosome, pericentric region
Molecular Function: protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding
Biological Process: meiosis; mitotic sister chromatid segregation; mitotic cell cycle checkpoint; RNA splicing; mitotic sister chromatid cohesion; stem cell maintenance; sister chromatid cohesion; DNA damage response, signal transduction; negative regulation of DNA endoreduplication; DNA repair; nuclear mRNA splicing, via spliceosome; response to radiation; mitotic spindle organization and biogenesis; cell division; gene expression; mitotic cell cycle
Disease: Cornelia De Lange Syndrome 2
Research Articles on SMC1A
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Product Notes
The SMC1A smc1a (Catalog #AAA125859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SMC1A-S957 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMC1A-S957 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:20 - 1:100. Researchers should empirically determine the suitability of the SMC1A smc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC1A-S957, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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