Rabbit anti-Human PERK Antibody | anti-PERK antibody

PERK Antibody

Cat. Num. AAA8503006

• Gene Names: EIF2AK3; PEK; WRS; PERK
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: PERK; PERK Antibody; EIF2AK3; DKFZp781H1925; HRI; PEK; WRS; anti-PERK antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1116

• Applicable Applications for anti-PERK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide of human PERK
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of MCF7 cellline, using PERK antibody.)

Related Product Information for anti-PERK antibody

PERK (protein kinase-like endoplasmic reticulum kinase) is an eIF2alpha kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2alpha to promote reduced translation. PERK-deficient mice have defects in pancreatic beta cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

Product Categories/Family for anti-PERK antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Harding, H. et al. (1999) Nature 397, 271-274. 2. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509. 3. Harding, H. et al. (2000) Mol. Cell 5, 897-904. 4. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

NCBI and Uniprot Product Information

• NCBI GI #: 134304838
• NCBI GeneID: 9451
• NCBI Accession #: NP_004827.4
• NCBI GenBank Nucleotide #: NM_004836.6
• UniProt Accession #: Q9NZJ5; O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9
• Molecular Weight: 125kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 alpha kinase 3
• NCBI Official Symbol: EIF2AK3
• NCBI Official Synonym Symbols: PEK; WRS; PERK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Gene Name: EIF2AK3
• UniProt Synonym Gene Names: PEK; PERK; HsPEK

NCBI Description

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Uniprot Description

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.