Rabbit anti-Human BMPR2 Antibody | anti-BMPR2 antibody

BMPR2, NT (BMPR2, PPH1, Bone morphogenetic protein receptor type-2, Bone morphogenetic protein receptor type II)

Cat. Num. AAA645975

• Gene Names: BMPR2; BMR2; PPH1; BMPR3; BRK-3; T-ALK; BMPR-II
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry
• Purity: Purified by ammomnium sulfate precipitation.
• Synonyms: BMPR2; NT (BMPR2; PPH1; Bone morphogenetic protein receptor type-2; Bone morphogenetic protein receptor type II); Anti -BMPR2; anti-BMPR2 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Specificity: Recognizes human BMPR2.
• Purity/Purification: Purified by ammomnium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 1.88mg/ml (varies by lot)

• Applicable Applications for anti-BMPR2 antibody: Western Blot (WB), Immunohistochemistry (IHC) (FFPE), Flow Cytometry (FC)
• Application Notes: Western Blot: 1:1000; Immunohistochemistry (FFPE): 1:10-1:50; Flow Cytometry: 1:10-1:50; Optimal dilutions to be determined by the researcher.
• Grade: Purified
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 28-59 in the N-terminal region of human BMPR2.
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human heart lysate (20ug/lane) using MBS645975. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 115kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MBS645975 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human kidney tissue reacted with MBS645975, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.)

Flow Cytometry (FC)

(Flow Cytometric analysis of HepG2 cells using MBS645975 (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BMPR2 antibody

BMPR2 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in BMPR2 have been associated with primary pulmonary hypertension.

Product Categories/Family for anti-BMPR2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 15451916
• NCBI GeneID: 659
• NCBI Accession #: NP_001195.2
• NCBI GenBank Nucleotide #: NM_001204.6
• UniProt Accession #: Q13873; Q16569; Q4ZG08; Q53SA5; Q585T8
• NCBI Official Full Name: bone morphogenetic protein receptor type-2
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type II (serine/threonine kinase)
• NCBI Official Symbol: BMPR2
• NCBI Official Synonym Symbols: BMR2; PPH1; BMPR3; BRK-3; T-ALK; BMPR-II
• NCBI Protein Information: bone morphogenetic protein receptor type-2; BMPR-2; BMP type-2 receptor; BMP type II receptor; type II activin receptor-like kinase; bone morphogenetic protein receptor type II; type II receptor for bone morphogenetic protein-4
• UniProt Protein Name: Bone morphogenetic protein receptor type-2
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: BMPR2
• UniProt Synonym Gene Names: PPH1; BMP type-2 receptor; BMPR-2; BMP type II receptor; BMPR-II
• UniProt Entry Name: BMPR2_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.

Catalytic activity: ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Cofactor: Magnesium or manganese

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Highly expressed in heart and liver.

Involvement in disease: Pulmonary hypertension, primary, 1 (PPH1) [MIM:178600]: A rare disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial pulmonary hypertension is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15Pulmonary venoocclusive disease (PVOD) [MIM:265450]: Rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14 Ref.16

Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.Contains 1 protein kinase domain.