Rabbit anti-Human Factor XIIIa Antibody | anti-F13A1 antibody

Factor XIIIa (Coagulation Factor XIIIA Chain)

Cat. Num. AAA4380891

• Gene Names: F13A1; F13A
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Factor XIIIa; Factor XIIIa (Coagulation Factor XIIIA Chain); Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; F13A; F13a1; Fibrin stabilizing factor; A subunit; Fibrinoligase; FSF; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase. plasma; anti-F13A1 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Clone Number: F13A1/3772R
• Specificity: It recognizes a protein of 83kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 732

• Applicable Applications for anti-F13A1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human placenta tissue (IHC).
• Immunogen: Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic and Secreted
• Hu-chromosome Location: 6p25.3-p24.3
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human placenta stained with Factor 13A Recombinant Rabbit Monoclonal Antibody (F13A1/3772R).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human placenta stained with Factor 13A Recombinant Rabbit Monoclonal Antibody (F13A1/3772R).)

References

Davie, EW. et al. 1991. The coagulation cascade: initiation, maintenance, and regulation. Biochemistry 30: 10363-10370.

NCBI and Uniprot Product Information

• NCBI GI #: 119395709
• NCBI GeneID: 2162
• NCBI Accession #: NP_000120.2
• NCBI GenBank Nucleotide #: NP_000120.2
• UniProt Accession #: P00488
• Molecular Weight: 83kDa
• NCBI Official Full Name: coagulation factor XIII A chain
• NCBI Official Synonym Full Names: coagulation factor XIII A chain
• NCBI Official Symbol: F13A1
• NCBI Official Synonym Symbols: F13A
• NCBI Protein Information: coagulation factor XIII A chain
• UniProt Protein Name: Coagulation factor XIII A chain
• Protein Family: Coagulation factor
• UniProt Gene Name: F13A1
• UniProt Synonym Gene Names: F13A; Coagulation factor XIIIa
• UniProt Entry Name: F13A_HUMAN

NCBI Description

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Uniprot Description

F13A1: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD). FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Belongs to the transglutaminase superfamily. Transglutaminase family.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 6p25.3-p24.3

Cellular Component: extracellular region

Molecular Function: protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: platelet activation; platelet degranulation; peptide cross-linking; blood coagulation

Disease: Thrombophilia Due To Thrombin Defect; Myocardial Infarction, Susceptibility To; Factor Xiii, A Subunit, Deficiency Of