Typical Testing Data/Standard Curve (for reference only)
Rabbit PDIA5 ELISA Kit | PDIA5 elisa kit
Rabbit PDIA5 (Protein Disulfide Isomerase A5) ELISA Kit
Gene Names
PDIA5; PDIR
Reactivity
Rabbit
Synonyms
PDIA5; Rabbit PDIA5 (Protein Disulfide Isomerase A5) ELISA Kit; PDIA5 elisa kit
Reactivity
Rabbit
Specificity
This kit recognizes natural and recombinant Rabbit PDIA5. No significant cross-reactivity or interference between Rabbit PDIA5 and analogues was observed.
Sequence Length
519
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDIA5 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit PDIA5 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDIA5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDIA5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDIA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PDIA5. You can calculate the concentration of PDIA5 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDIA5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDIA5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDIA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PDIA5. You can calculate the concentration of PDIA5 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,124 Da
NCBI Official Full Name
protein disulfide-isomerase A5
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 5
NCBI Official Symbol
PDIA5
NCBI Official Synonym Symbols
PDIR
NCBI Protein Information
protein disulfide-isomerase A5; protein disulfide isomerase-associated 5; protein disulfide isomerase-related protein
UniProt Protein Name
Protein disulfide-isomerase A5
Protein Family
UniProt Gene Name
PDIA5
UniProt Synonym Gene Names
PDIR
UniProt Entry Name
PDIA5_HUMAN
Uniprot Description
PDIA5: Belongs to the protein disulfide isomerase family.
Protein type: Oxidoreductase; Isomerase; Endoplasmic reticulum; Chaperone; EC 5.3.4.1
Chromosomal Location of Human Ortholog: 3q21.1
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; endoplasmic reticulum lumen
Molecular Function: protein disulfide isomerase activity; oxidoreductase activity
Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; protein folding; unfolded protein response; cell redox homeostasis
Research Articles on PDIA5
Similar Products
Product Notes
The Rabbit PDIA5 pdia5 (Catalog #AAA2513868) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513868 ELISA Kit recognizes Rabbit PDIA5. It is sometimes possible for the material contained within the vial of "PDIA5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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