Rabbit Osteocalcin/Bone Gla Protein (OT/BGP) ELISA Kit | OT/BGP elisa kit

Rabbit Osteocalcin/Bone Gla Protein (OT/BGP) ELISA Kit

Cat. Num. AAA34151

• Gene Names: BGLAP; OC; BGP; OCN
• Reactivity: Rabbit
• Synonyms: Osteocalcin/Bone Gla Protein (OT/BGP); Rabbit Osteocalcin/Bone Gla Protein (OT/BGP) ELISA Kit; OT/BGP elisa kit

• Reactivity: Rabbit

• Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids
• Detection Range: 0.625 ng/ml ~ 20 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rabbit OT/BGP in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Rabbit OT/BGP concentration. The concentration of Rabbit OT/BGP in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rabbit OT/BGP in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Rabbit OT/BGP concentration. The concentration of Rabbit OT/BGP in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OT/BGP elisa kit

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of specimens. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the specimen.

NCBI and Uniprot Product Information

• NCBI GI #: 40316933
• NCBI GeneID: 632
• NCBI Accession #: NP_954642.1
• NCBI GenBank Nucleotide #: NM_199173.4
• UniProt Accession #: P02818; Q5TCK6
• Molecular Weight: 10,963 Da
• NCBI Official Full Name: osteocalcin preproprotein
• NCBI Official Synonym Full Names: bone gamma-carboxyglutamate (gla) protein
• NCBI Official Symbol: BGLAP
• NCBI Official Synonym Symbols: OC; BGP; OCN
• NCBI Protein Information: osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• UniProt Protein Name: Osteocalcin
• UniProt Gene Name: BGLAP
• UniProt Synonym Gene Names: BGP
• UniProt Entry Name: OSTCN_HUMAN

NCBI Description

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

Uniprot Description

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon

Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity

Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; response to ethanol; regulation of osteoclast differentiation; response to vitamin D; response to vitamin K; response to zinc ion; response to mechanical stimulus; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization