Typical Testing Data/Standard Curve (for reference only)
Rabbit EGR4 ELISA Kit | EGR4 elisa kit
Rabbit EGR4 (Early Growth Response Protein 4) ELISA Kit
Gene Names
EGR4; NGFIC; NGFI-C; PAT133
Reactivity
Rabbit
Synonyms
EGR4; Rabbit EGR4 (Early Growth Response Protein 4) ELISA Kit; EGR4 elisa kit
Reactivity
Rabbit
Specificity
This kit recognizes natural and recombinant Rabbit EGR4. No significant cross-reactivity or interference between Rabbit EGR4 and analogues was observed.
Sequence Length
589
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EGR4 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit EGR4 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EGR4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EGR4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EGR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EGR4. You can calculate the concentration of EGR4 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EGR4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EGR4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EGR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EGR4. You can calculate the concentration of EGR4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,623 Da
NCBI Official Full Name
early growth response protein 4
NCBI Official Synonym Full Names
early growth response 4
NCBI Official Symbol
EGR4
NCBI Official Synonym Symbols
NGFIC; NGFI-C; PAT133
NCBI Protein Information
early growth response protein 4; AT133; EGR-4
UniProt Protein Name
Early growth response protein 4
Protein Family
UniProt Gene Name
EGR4
UniProt Synonym Gene Names
EGR-4
UniProt Entry Name
EGR4_HUMAN
Uniprot Description
EGR4: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. Belongs to the EGR C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 2p13
Cellular Component: cytoplasm; nucleus
Molecular Function: DNA binding; metal ion binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of apoptosis
Research Articles on EGR4
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Product Notes
The Rabbit EGR4 egr4 (Catalog #AAA2505545) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505545 ELISA Kit recognizes Rabbit EGR4. It is sometimes possible for the material contained within the vial of "EGR4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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