Rabbit Aggrecan (AGC) ELISA Kit | AGC elisa kit

Rabbit Aggrecan (AGC) ELISA Kit

Cat. Num. AAA2703880

• Gene Names: ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
• Reactivity: Rabbit
• Synonyms: Aggrecan (AGC); Rabbit Aggrecan (AGC) ELISA Kit; ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1; AGC elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aggrecan (AGC).; No significant cross-reactivity or interference between Aggrecan (AGC) and analogues was observed.
• Sequence Length: 2,316

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.224ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aggrecan (AGC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aggrecan (AGC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aggrecan (AGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aggrecan (AGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aggrecan (AGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22209083
• NCBI GeneID: 176
• UniProt Accession #: P16112; Q13650; Q9UCD3; Q9UCP4; Q9UCP5; Q9UDE0
• Molecular Weight: 239,235 Da
• NCBI Official Full Name: Aggrecan
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: ACAN
• NCBI Official Synonym Symbols: AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
• NCBI Protein Information: aggrecan core protein
• UniProt Protein Name: Aggrecan core protein
• UniProt Gene Name: ACAN
• UniProt Synonym Gene Names: AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.