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Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen I antibody.)

Rabbit Collagen I Antibody | anti-COL1A1 antibody

Collagen I Antibody

Gene Names
COL1A2; OI4; EDSCV; EDSARTH2
Reactivity
Human, Mouse, Rat
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Collagen I; Collagen I Antibody; Collagen alpha-2(I) chain; Alpha-2 type I collagen; COL1A2; anti-COL1A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
Isotype
IgG
Specificity
Collagen I antibody detects endogenous levels of total Collagen I protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1366
Applicable Applications for anti-COL1A1 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from N-terminal of human Collagen I.
Preparation and Storage
Store at-20°C for 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen I antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen I antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells, using Collagen I antibody.)

Immunofluorescence (IF) (Immunofluorescence analysis of NIH/3T3 cells, using Collagen I antibody.)
References
Masatoshi Jinnin, Nucleic Acids Res., Mar 2005; 33: 1337-1351. B. Stefanovic, Nucleic Acids Res., Jan 2000; 28: 641-647. DE Birk, J. Cell Biol., Mar 1988; 106: 999. Christina Meyer, J. Biol. Chem., Nov 2002; 277: 45400-45407.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-2(I) chain
NCBI Official Synonym Full Names
collagen type I alpha 2 chain
NCBI Official Symbol
COL1A2
NCBI Official Synonym Symbols
OI4; EDSCV; EDSARTH2
NCBI Protein Information
collagen alpha-2(I) chain
UniProt Protein Name
Collagen alpha-2(I) chain
Protein Family
UniProt Gene Name
COL1A2

NCBI Description

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Uniprot Description

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Research Articles on COL1A1

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Product Notes

The COL1A1 col1a2 (Catalog #AAA854891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen I Antibody reacts with Human, Mouse, Rat Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen I can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the COL1A1 col1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen I, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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