Rabbit Cyclin B1 Antibody | anti-CCNB1 antibody

Cyclin B1 (G2- & M-phase Cyclin)

Cat. Num. AAA4381373

• Gene Names: CCNB1; CCNB
• Reactivity: Human, Mouse, Hamster
• Applications: ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cyclin B1; Cyclin B1 (G2- & M-phase Cyclin); CCNB; CCNB1; CCNB1_HUMAN; G2 Mitotic Specific Cyclin B1; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Hamster
• Isotype: IgG
• Clone Number: CCNB1/2978R
• Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 433

• Applicable Applications for anti-CCNB1 antibody: ELISA (EIA)
• Application Notes: ELISA: For coating, order Ab without BSA
• Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
• Immunogen: Recombinant full-length human CCNB1 protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 5q13.2
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified Cyclin B1 Rabbit Recombinant Monoclonal Antibody (CCNB1/2978R). Confirmation of Integrity and Purity of Antibody.)

References

1. Minemoto Y et al. Exp Cell Res 262:37-48 (2001). 2. Garner AP et al. Oncogene 21:8089-104 (2002)

NCBI and Uniprot Product Information

• NCBI GI #: 14327896
• NCBI GeneID: 891
• NCBI Accession #: NP_114172.1
• NCBI GenBank Nucleotide #: NP_114172.1
• UniProt Accession #: P14635
• Molecular Weight: 55-62kDa
• NCBI Official Full Name: G2/mitotic-specific cyclin-B1 isoform 1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1
• UniProt Protein Name: G2/mitotic-specific cyclin-B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB1
• UniProt Synonym Gene Names: CCNB
• UniProt Entry Name: CCNB1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; patched binding; histone kinase activity; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; tissue regeneration; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle