QPRT recombinant protein

Recombinant Human QPRT Protein

Cat. Num. AAA8249687

• Gene Names: QPRT; QPRTase; HEL-S-90n
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: QPRT; Recombinant Human QPRT Protein; Nicotinate-nucleotide pyrophosphorylase [carboxylating]; Quinolinate phosphoribosyltransferase [decarboxylating]; QAPRTase; QPRTase; QPRT recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Sequence: MGSSHHHHHH SSGLVPRGSH MDAEGLALLL PPVTLAALVD SWLREDCPGL NYAALVSGAG PSQAALWAKS PGILAGQPFF DAIFTQLNCQ VSWFLPEGSK LVPVARVAEV RGPAHCLLLG ERVALNTLAR CSGIASAAAA AVEAARGAGW TGHVAGTRKT TPGFRLVEKY GLLVGGAASH RYDLGGLVMV KDNHVVAAGG VEKAVRAARQ AADFALKVEV ECSSLQEAVQ AAEAGADLVL LDNFKPEELH PTATVLKAQF PSVAVEASGG ITLDNLPQFC GPHIDVISMG MLTQAAPALD FSLKLFAKEV APVPKIH
• Sequence Length: 152

• Applicable Applications for QPRT recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for QPRT recombinant protein

Recombinant Human QPRT Protein is produced by our E Coli expression system and the target gene encoding Met1-His297 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 970949423
• NCBI GeneID: 23475
• NCBI Accession #: NP_001305178.1
• NCBI GenBank Nucleotide #: NM_001318249.1
• UniProt Accession #: Q15274; Q53XW7; Q96G22; Q9BSG6
• Molecular Weight: 30,846 Da
• NCBI Official Full Name: nicotinate-nucleotide pyrophosphorylase
• NCBI Official Synonym Full Names: quinolinate phosphoribosyltransferase
• NCBI Official Symbol: QPRT
• NCBI Official Synonym Symbols: QPRTase; HEL-S-90n
• NCBI Protein Information: nicotinate-nucleotide pyrophosphorylase [carboxylating]
• UniProt Protein Name: Nicotinate-nucleotide pyrophosphorylase [carboxylating]
• Protein Family: Nicotinate-nucleotide pyrophosphorylase
• UniProt Gene Name: QPRT
• UniProt Synonym Gene Names: QAPRTase; QPRTase
• UniProt Entry Name: NADC_HUMAN

NCBI Description

This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

QPRT: Involved in the catabolism of quinolinic acid (QA). Belongs to the NadC/ModD family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.2.19; Transferase

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasm; cytosol

Molecular Function: nicotinate-nucleotide diphosphorylase (carboxylating) activity; protein homodimerization activity

Biological Process: NAD biosynthetic process; NAD metabolic process; protein oligomerization