Peptide YY (PYY) Recombinant Protein | PYY recombinant protein

Recombinant Peptide YY (PYY)

Cat. Num. AAA2018520

• Gene Names: PYY; PYY1; PYY-I
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peptide YY (PYY); Recombinant Peptide YY (PYY); PYY recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: Residues: Ile31~Trp97 (Accession # P10082), with two N-terminal Tags, His-tag and T7-tag. The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IKPEAPREDA SPEELNRYYA SLRHYLNLVT RQRYGKRDGP DTLLSKTFFP DGEDRPVRSR SEGPDLW
• Sequence Length: 97

• Applicable Applications for PYY recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 300068956
• NCBI GeneID: 5697
• NCBI Accession #: NP_004151.3
• NCBI GenBank Nucleotide #: NM_004160.4
• UniProt Accession #: P10082; Q5U5Q6; Q6FGH8
• Molecular Weight: 11.7kDa
• NCBI Official Full Name: peptide YY preproprotein
• NCBI Official Synonym Full Names: peptide YY
• NCBI Official Symbol: PYY
• NCBI Official Synonym Symbols: PYY1; PYY-I
• NCBI Protein Information: peptide YY; prepro-PYY; peptide tyrosine tyrosine
• UniProt Protein Name: Peptide YY
• Protein Family: Peptide
• UniProt Gene Name: PYY
• UniProt Synonym Gene Names: PYY
• UniProt Entry Name: PYY_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010]

Uniprot Description

PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; G-protein-coupled receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; neuropeptide signaling pathway; eating behavior; regulation of appetite; digestion; feeding behavior; cytoskeleton organization and biogenesis; cell motility

Disease: Obesity