Glycogen Phosphorylase, Liver (PYGL) Recombinant Protein | PYGL recombinant protein

Recombinant Glycogen Phosphorylase, Liver (PYGL)

Cat. Num. AAA2010100

• Gene Names: PYGL; GSD6
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 97%
• Synonyms: Glycogen Phosphorylase; Liver (PYGL); Recombinant Glycogen Phosphorylase; PYGL recombinant protein

• Host: E Coli
• Purity/Purification: > 97%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; NDPMVGSK LKVIFLENYR VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM AEEAGEENLF IFGMRIDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF FSPKQPDLFK DIINMLFYHD RFKVFADYEA YVKCQDKVSQ LYMNPKAWNT MVLKNIAASG KFSSDRTIKE
• Sequence Length: 813

• Applicable Applications for PYGL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn633~Glu820 (Accession # P06737) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 255653002
• NCBI GeneID: 5836
• NCBI Accession #: NP_001157412.1
• NCBI GenBank Nucleotide #: NM_001163940.1
• UniProt Accession #: P06737; O60567; O60752; O60913; Q501V9; Q641R5; Q96G82; A6NDQ4; B4DUB7; F5H816
• Molecular Weight: 22.6kDa
• NCBI Official Full Name: glycogen phosphorylase, liver form isoform 2
• NCBI Official Synonym Full Names: phosphorylase, glycogen, liver
• NCBI Official Symbol: PYGL
• NCBI Official Synonym Symbols: GSD6
• NCBI Protein Information: glycogen phosphorylase, liver form
• UniProt Protein Name: Glycogen phosphorylase, liver form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGL
• UniProt Entry Name: PYGL_HUMAN

NCBI Description

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

PYGL: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. Belongs to the glycogen phosphorylase family.

Protein type: Transferase; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 14q21-q22

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: glycogen phosphorylase activity; protein homodimerization activity; bile acid binding; purine binding; drug binding; vitamin binding; ATP binding; glucose binding; pyridoxal phosphate binding; AMP binding

Biological Process: glycogen metabolic process; glycogen catabolic process; carbohydrate metabolic process; 5-phosphoribose 1-diphosphate biosynthetic process; glucose metabolic process; pathogenesis; glucose homeostasis

Disease: Glycogen Storage Disease Vi