Bovine Glycogen Phosphorylase, Brain ELISA Kit | PYGB elisa kit

Bovine Glycogen Phosphorylase, Brain ELISA Kit

Cat. Num. AAA744709

• Gene Names: PYGB; GPBB
• Reactivity: Bovine
• Synonyms: Glycogen Phosphorylase; Brain; Bovine Glycogen Phosphorylase; Brain ELISA Kit; PYGB elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PYGB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PYGB. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PYGB elisa kit

Principle of the Assay: PYGB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYGB antibody and an PYGB-HRP conjugate. The assay sample and buffer are incubated together with PYGB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYGB concentration since PYGB from samples and PYGB-HRP conjugate compete for the anti-PYGB antibody binding site. Since the number of sites is limited, as more sites are occupied by PYGB from the sample, fewer sites are left to bind PYGB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYGB concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21361370
• NCBI GeneID: 5834
• NCBI Accession #: NP_002853.2
• NCBI GenBank Nucleotide #: NM_002862.3
• Molecular Weight: 96,696 Da
• NCBI Official Full Name: glycogen phosphorylase, brain form
• NCBI Official Synonym Full Names: phosphorylase, glycogen; brain
• NCBI Official Symbol: PYGB
• NCBI Official Synonym Symbols: GPBB
• NCBI Protein Information: glycogen phosphorylase, brain form; glycogen phosphorylase B
• UniProt Protein Name: Glycogen phosphorylase, brain form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGB
• UniProt Entry Name: PYGB_HUMAN

NCBI Description

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1; Kinase, other; Transferase; Phosphorylase

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis