Human Peroxidasin homolog ELISA Kit | PXDN elisa kit

Human Peroxidasin homolog ELISA Kit

Cat. Num. AAA763931

• Gene Names: PXDN; PXN; VPO; MG50; PRG2; COPOA; D2S448; D2S448E
• Reactivity: Human
• Synonyms: Peroxidasin homolog; Human Peroxidasin homolog ELISA Kit; PXDN/Peroxidasin homolog/p53-responsive gene 2 protein/Melanoma-associated antigen MG50/Vascular peroxidase 1/MG50/VPO/VPO1; PXDN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PXDN. No significant cross-reactivity or interference between PXDN and analogues was observed.
• Sequence Length: 1479

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for PXDN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 109150416
• NCBI GeneID: 7837
• NCBI Accession #: NP_036425.1
• NCBI GenBank Nucleotide #: NM_012293.2
• Molecular Weight: 80,329 Da
• NCBI Official Full Name: peroxidasin homolog
• NCBI Official Synonym Full Names: peroxidasin
• NCBI Official Symbol: PXDN
• NCBI Official Synonym Symbols: PXN; VPO; MG50; PRG2; COPOA; D2S448; D2S448E
• NCBI Protein Information: peroxidasin homolog
• UniProt Protein Name: Peroxidasin homolog
• Protein Family: Peroxidasin
• UniProt Gene Name: PXDN
• UniProt Synonym Gene Names: KIAA0230; MG50; PRG2; VPO; VPO1
• UniProt Entry Name: PXDN_HUMAN

NCBI Description

This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]

Uniprot Description

PXDN: Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation. Belongs to the peroxidase family. XPO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 1.11.1.7; Secreted; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: endoplasmic reticulum; extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; heme binding; interleukin-1 receptor antagonist activity; metal ion binding; peroxidase activity

Biological Process: extracellular matrix organization and biogenesis; hydrogen peroxide catabolic process; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to oxidative stress

Disease: Corneal Opacification With Other Ocular Anomalies